kir6 1  (Bioss)

Journal: Journal of Neuroinflammation

Article Title: Oral administration of the K ATP channel opener diazoxide ameliorates disease progression in a murine model of multiple sclerosis

doi: 10.1186/1742-2094-8-149

Figure Lengend Snippet: Western blotting show expression of both Kir6.1 and Kir6.2 K ATP channel pore-forming subunits in unstimulated and LPS/IFNγ-stimulated BV-2 cells (A, left) and microglial primary cultures (A, Right). Staining for the microglial cell membrane marker CD11b (B and E) and the K ATP channel subunits Kir 6.1 (C) or Kir 6.2 (F) showed colocalization in BV-2 microglia, indicating the expression of the K ATP channel at the cytoplasmic membrane (D and G, white arrows) . Control: unstimulated cells; L+I: cells stimulated with LPS and IFNγ. Scale bar = 30 μm.

Article Snippet: After washing in Tris-buffered saline (TBS: 20 mM Tris, 0.15 M NaCl, pH 7.5) for 5 min, dipping in methanol for 10 s and drying in air, the membranes were incubated with the following primary antibodies overnight at 4°C: polyclonal rabbit anti-Kir 6.1 or polyclonal rabbit anti-Kir 6.2 (both 1:500, Alomone, Jerusalem, Israel), polyclonal rabbit anti-inducible nitric oxide synthase (iNOS) (1:200, Millipore, Bedford, MA, USA), polyclonal rabbit anti-cyclooxygenase-2 (1:2000, Santa Cruz Biotechnology, St. Cruz, CA, USA) and monoclonal mouse anti-β-actin (1:50000, Sigma-Aldrich, St. Louis, MO, USA) diluted in immunoblot buffer (TBS containing 0.05% Tween-20 and 5% non-fat dry milk).

Techniques: Western Blot, Expressing, Staining, Marker

Journal: The Journal of Veterinary Medical Science

Article Title: ATP-dependent potassium channels contribute to motor regulation of esophageal striated muscle in rats

doi: 10.1292/jvms.19-0197

Figure Lengend Snippet: Expression of subunits of K ATP channels in the rat esophagus determined by RT-PCR. Homogenized samples from the rat tissues were used for RT-PCR. Amplified products of mRNA of Kir6.1, Kir6.2, SUR1, SUR2A and SUR2B were detected in appropriate sizes ( n =3).

Article Snippet: The sections were then incubated with 2% normal goat serum at room temperature for 30 min. After rinsing in PBS, the sections were incubated with anti-SUR1 polyclonal antibody (catalog number: bs-3641R; lot number: 9B21M9; Bioss, Boston, MA, U.S.A.) diluted 1:100 or with anti-Kir6.1 polyclonal antibody (catalog number: bs-6468R; lot number: AF02227906; Bioss) diluted 1:100 at 4°C overnight.

Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification

Journal: The Journal of Veterinary Medical Science

Article Title: ATP-dependent potassium channels contribute to motor regulation of esophageal striated muscle in rats

doi: 10.1292/jvms.19-0197

Figure Lengend Snippet: Histological examinations of the rat esophagus with paraffin section. (A) Kir6.1 immunoreactivity was observed mainly in the muscular layer in the rat esophagus. (B) The control staining using a normal rabbit IgG instead of antibody for K ATP channel. No specific staining was observed. L shows the lumen. Scale bar=100 µ m.

Article Snippet: The sections were then incubated with 2% normal goat serum at room temperature for 30 min. After rinsing in PBS, the sections were incubated with anti-SUR1 polyclonal antibody (catalog number: bs-3641R; lot number: 9B21M9; Bioss, Boston, MA, U.S.A.) diluted 1:100 or with anti-Kir6.1 polyclonal antibody (catalog number: bs-6468R; lot number: AF02227906; Bioss) diluted 1:100 at 4°C overnight.

Techniques: Paraffin Section, Staining

Journal: The Journal of Veterinary Medical Science

Article Title: ATP-dependent potassium channels contribute to motor regulation of esophageal striated muscle in rats

doi: 10.1292/jvms.19-0197

Figure Lengend Snippet: Histological examinations of the rat esophagus with cryostat section. The muscular layer of the rat esophagus showed immunoreactivity by antibodies not only for Kir6.1 (A) but also for SUR1 (B). (C) The control staining using a normal rabbit IgG instead of antibody for K ATP channel. No specific staining was observed. L shows the lumen. Scale bar=100 µ m.

Article Snippet: The sections were then incubated with 2% normal goat serum at room temperature for 30 min. After rinsing in PBS, the sections were incubated with anti-SUR1 polyclonal antibody (catalog number: bs-3641R; lot number: 9B21M9; Bioss, Boston, MA, U.S.A.) diluted 1:100 or with anti-Kir6.1 polyclonal antibody (catalog number: bs-6468R; lot number: AF02227906; Bioss) diluted 1:100 at 4°C overnight.

Techniques: Staining